Abstract
Fluorescence spectroscopy is a useful technique for investigating the interaction of DNA-binding proteins with DNA. Generally, use is made of the intrinsic fluorescence of the protein arising from the aromatic amino acids, which is frequently perturbed in a DNA-protein complex (see Chapter 33). In some cases, however, changes in the intrinsic fluorescence emission of a protein arising from its interaction with nucleic acid may not be detectable. For example, if tryptophan and/or tyrosine residues are not located in the proximity of the DNA-binding site, the emission spectrum may not be perturbed by the interaction. Furthermore, in the presence of a large number of tryptophan and tyrosine residues, a relatively small perturbation in the overall emission spectrum brought about by DNA binding may be masked.
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© 2001 Humana Press Inc., Totowa, NJ
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Taylor, I.A., Kneale, G.G. (2001). A Competition Assay for DNA Binding Using the Fluorescent Probe ANS. In: Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 148. Humana Press. https://doi.org/10.1385/1-59259-208-2:265
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DOI: https://doi.org/10.1385/1-59259-208-2:265
Publisher Name: Humana Press
Print ISBN: 978-0-89603-625-3
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