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High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling

  • Kent McDonald
Part of the Methods in Molecular Biology™ book series (MIMB, volume 117)

Abstract

What is high-pressure freezing (HPF)? HPF is a method of specimen preparation for electron microscopy (EM) that freezes noncryoprotected samples up to 0.5 mm in thickness without significant ice crystal damage. Other freezing methods are limited to 1/100 (plunge and impact freezing) to 1/10 (propane jet freezing) that sample thickness. With HPF you are working in the realm of whole cells and tissues instead of small cells or cell cortical regions. For more details on the theory of how HPF works, and some examples of its application, there are several reviews available (1-4).

Keywords

Uranyl Acetate Microfuge Tube Aluminum Block Paper Point Conventional Transmission Electron Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Kent McDonald
    • 1
  1. 1.Electron Microscope LabUniversity of CaliforniaBerkeley

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