Abstract
The fission yeast Schizosaccharomyces pombe serves as a model system for investigating a wide variety of problems in eukaryotic cellular and molecular biology. Although probably most widely studied in relation to the control of the eukaryotic cell cycle (1) and the events of cell division (2,3), S. pombe has also received significant attention in studies of protein trafficking and secretion (4), cell wall biosynthesis (5), cellular and molecular aspects of meiosis, genetic recombination, and spore formation (6), signal transduction pathways and control of gene expression (7-10) and cell growth (11,12), cell morphology (13,14) and the cytoskeleton (15,16). In many ways we can think of S. pombe as essentially a typical eukaryote trapped inside a cell wall, and the resulting constraints on cell morphology confer on it a stereotyped cellular architecture, well suited to ultrastructural analysis. The details of this architecture at both the light- and electron-microscopic level can be found in reference (17); here we will only highlight certain features.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Nurse, P. (1990) Universal control mechanism regulating onset of M-phase Nature 344, 503–508.
Yanagida, M. (1989) Gene products required for chromosome separation. J Cell Sci. Suppl. 12, 213–29.
Simanis, V. (1995) The control of septum formation and cytokinesis in fissionyeast. Semin. Cell Biol. 6, 79–87.
Armstrong, J., Pidoux, A., Bowden, S., Craighead, M., Bone, N., and Robinson, E. (1994) The ypt proteins of Schizosaccharomycespombe. Biochem. Soc. Trans. 22, 460–463.
Arellano, M., Duran, A., and Perez, P. (1996) Rho1 GTPase activates the (1-3) beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis. EMBO J. 13, 4584–4591.
Egel, R. (1989) Mating-type genes, meiosis, and sporulation. in Molecular Biology of the Fission Yeast. Nasim, A., Young, P., and Johnson, B. F., eds. Academic Press, San Diego, pp. 31–73.
Hughes, D. A. (1995) Control of signal transduction and morphogenesis by Ras. Semin. Cell Biol. 6, 89–94.
Wilkinson, M. G., Samuels, M., Takeda, T., Toone, W. M., Shieh, J. C., Toda, T., Millar, J. B., and Jones. N. (1996) The Atf 1 transcription factor is a target for the Sty1 stress-xactivated MAP kinase pathway in fission yeast. Genes Dev. 10,2289–2301.
Sturm, S., and Okayama, H. (1996) Domains determining the functional distinction of the fission yeast cell cycle “start” molecules Res1 and Res2. Mol. Biol. Cell 7, 1967–1976.
Zhu, Y., Takeda, T., Whitehall, S., Peat, N., and Jones, N. (1997) Functional characterization of the fission yeast Start-specific transcription factor Res2. EMBO J. 16,1023–1034.
Streiblova, E., and Wolf, A. (1972) Cell wall growth during the cell cycle of Schizosaccharomyces pombe. Zeitschrift f. Allg. Mikrobiologie 12, 673–684.
Mitchison, J. M., and Nurse, P. (1985) Growth in cell length in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 75, 357–76.
Verde, F., Mata, J., and Nurse, P. (1995) Fission yeast cell morphogenesis: identification of new genes and analysis of their roles during the cell cycle. J. of Cell Biology 131, 1529–38.
Snell, V., and Nurse, P. (1993) Investigations into the control of cell form and polarity: the use of morphological mutants in fission yeast. Dev. Suppl. 289–299.
Marks, J., Hagan, I. M., and Hyams, J. S. (1986) Growth polarity and cytokinesis in fission yeast: the role of the cytoskeleton. J. Cell Sci. Suppl. 5, 229–41.
Hagan, I. M., and Hyams, J. S., (1988) The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 89, 343–357.
Robinow, C. F., and Hyams, J. S. (1989) General cytology of fission yeasts. in Molecular Biology of the Fission Yeast. Nasim, A., Young, P., and Johnson, B. F., eds. Academic Press, San Diego, pp. 273–330.
Chang, F., and Nurse, EP. (1996) How fission yeast fission in the middle. Cell 84, 191–194.
Tanaka, K., and Kanbe, T.(1986) Mitosis in the fission yeast S. pombe as revealed by freeze-substitution electron microscopy. J. Cell Sci. 80, 253–268.
Yamamoto, M. (1996) The molecular control mechanisms of meiosis in fission yeast. Trends Biochem. Sci. 21,18–22.
Nielsen, O., and Davey, J. (1995) Pheromone communication in the fission yeast Schizosaccharomyces pombe. Semin. Cell Biol. 6, 95–104.
Stern, B., and Nurse, P. (1997) Fission yeast pheromone blocks S-phase progression by inhibiting the G1 cyclin B-p34cdc2 kinase. EMBO J. 16, 534–544.
Fukui, Y., Kaziro, Y., and Yamamoto, M. (1986) Mating-pheromone-like diffusible factor released by Schizosaccharomyces pombe. EMBO J. 5, 1991–1993.
Leupold U. (1987) Sex appeal in fission yeast. Curr. Genet. 12, 543–545.
Chikashige, Y., Ding, D. Q., Funabiki, H., Haraguchi, Y., Mashiko, S., Yanagida, M., and Hiraoka, Y. (1994) Telomere-led premeiotic chromosome movement in fission yeast. Science 264, 270–273.
Hayles, J., and Nurse, P. (1992) Genetics of the fission yeast Schizosaccharomyces pombe. Ann. Rev. Genet. 26, 373–402.
Hoheisel, J. D., Maier, E., Mott, R., McCarthy, L., Grigoriev, A. V., Schalkwyk, L. C., Nizetic, D., Francis, F., and Lehrach, H. (1993) High-resolution cosmid and P1-maps spanning the 14MB genome of the fission yeast S. pombe. Cell 73,109–120.
Grimm, C., Kohli, J., Murray, J., and Maundrell, K. (1988) Genetic engineering in Schizosaccharomyces pombe a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol. Gen. Genet. 215, 81–86.
Maundrell, K. (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene. 123, 127–130.
Sipiczki, M. (1989) Taxonomy and phylogenesis, in Molecular Biology of the Fission Yeast. Nasim, A., Young, P., and Johnson, B. F., eds., Academic Press, San Diego, pp. 431–452.
Kaufer, N. F., Simanis, V., and Nurse, P. (1985) Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence. Nature 318, 78–80.
Dubey, D. D., Kim, S. M., Todorov, I. T., and Huberman, J. A. (1996) Large, complex modular structure of a fission yeast DNA replication origin. Curr. Biol. 6,467–473.
Nakaseko, Y., Adachi, Y., Funakashi, S., Niwa, O., and Yanagida, M. (1986) Chromosome walking shows highly homologous repetitive sequence present in all the centromere regions of fission yeast. EMBO J. 5, 1011–1021.
Moreno, S., Klar, A., and Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Meth. Enzymol. 194, 795–83.
Ayscough, K., Hajibagheri, N. M., Watson, R., and Warren, G. (1993) Stacking of Golgi cisternae in Schizosaccharomyces pombe requires intact microtubules. J. Cell Sci. 106, 1227–1237.
Ruhrberg C., Hajibagheri, M. A. N., Simon, M., Dooley, T., and Watt, F. M. (1996). Envoplakin, a novel precursor of the cornified envelope that has homo-logy to desmoplakin. J. CellBiol. 134(3), 715–29, 1996.
Ruhrberg, C., Hajibagheri, M. A. N., Parry, D. M., and Watt, F. M. (1997) Periplakin, a novel component cornified envelopes and desmosomes that belongs to the plakin family and forms complexes with envoplakin. J. Cell Biol. 139,1835–1849.
Chappell, T. J., Hajibagheri, M. A., Ayscough, K., Pierce, M., and Warren, G. (1994) Localization of an 1, 2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe. Mol. Biol. Cell 5, 519–528.
Goode, N. T, Hajibagheri, M. A. N., Warren, G. B., and Parker, P. J. (1994) Expression of mammalian protein kinase C in Schizosaccharomyces pombe: isotype specific induction of growth arrest, vesicle formation and endocytosis. Mol. Biol. Cell 5, 907–920.
Goode, N. T., Hajibagheri, M. A. N., and Parker, P. J. (1995) Protein Kinase C (PKC)-induced PKC Down. J. Biol. Chem. 27, 2669–2673.
Dittie, A., Hajibagheri, M. A. N., and Tooze, S. (1996) The Ap-1 adaptor complex binds to immature secretory granules from PC 12 cells and is regulated by ADP-ribosylation factor. J. Cell Biol. 132, 523–536.
Whitehouse, C., Burchell, J., Gschmeissner, S., Brockhausen, I., Lloyd, K. O., Taylor-Papadimitriou, J. (1997). A transfected sialyltransferase that is elevated in breast cancer and localizes to the medial/trans Golgi apparatus inhibits the development of core-2-based O-glycans.J. CellBiol. 137(6), 1229–41.
James, C., Gschmeissner, S., Fraser, A., Evan, G. I. (1997) CED-4 induces chro-matin condensation in Schizosaccharomyces pombe and is inhibited by direct physical association with CED-9. Curr. Biol. 7(4), 246–52.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Hajibagheri, M.N., Sawin, K., Gschmeissner, S., Blight, K., Upton, C. (1999). Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study. In: Nasser Hajibagheri, M.A. (eds) Electron Microscopy Methods and Protocols. Methods in Molecular Biology™, vol 117. Humana Press. https://doi.org/10.1385/1-59259-201-5:183
Download citation
DOI: https://doi.org/10.1385/1-59259-201-5:183
Publisher Name: Humana Press
Print ISBN: 978-0-89603-640-6
Online ISBN: 978-1-59259-201-2
eBook Packages: Springer Protocols