Electron Microscopic Enzyme Cytochemistry

  • Nobukazu Araki
  • Tanenori Hatae
Part of the Methods in Molecular Biology™ book series (MIMB, volume 117)


The primary aim of enzyme cytochemistry is elucidation of intracellular localization of enzymes. Enzyme cytochemical reactions are based on the enzymatic conversion of a substrate, followed by the deposition of electron dense products at the enzyme site. So, enzyme cytochemistry reveals not only the location of enzyme but also the intensity of its catalytic activity. The data obtained by enzyme cytochemistry can be easily compared to biochemical enzyme assay in test tubes. Immunocytochemistry detects the enzyme molecule itself, but gives no information about enzyme activity. Instead, immunocytochemistry is more correlative to immnological techniques such as Western blotting. Thus, enzyme cytochemistry shows distinct biological significance from immunocytochemistry.


Incubation Medium Acid Phosphatase Activity Propylene Oxide Lead Phosphate Pipe Buffer 
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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Nobukazu Araki
    • 1
  • Tanenori Hatae
    • 1
  1. 1.Department of AnatomyKagawa Medical UniversityKagawaJapan

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