Abstract
This chapter is aimed at those who have not previously done any processing for electron microscopy (EM). It deals with basic preparation of many different types of mammalian material for ultrastructural examination; for processing of plant material (see Hall and Hawes, ref. 1). The material to be processed may be cell suspensions, particulates, monolayer cultures, or tissue derived from organs. The former three must initially be processed differently from the latter.
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References
Hall, J. L. and Hawes, C. (eds.) (1991) Electron Microscopy of Plant Cells, Academic, London, England.
Glauert, A. M. (1974) Fixation, dehydration and embedding of biological specimens, in Practical Methods in Electron Microscopy, vol. 3, part I, North-Holland, Amsterdam.
Reid, N. (1974) Ultramicrotomy, in Practical Methods in Electron Microscopy, vol. 3, part II, North-Holland, Amsterdam.
Robards, A. W. and Wilson, A. J. (1993) Basic Biological Preparation Techniques for TEM, in Procedures in Electron Microscopy, Wiley, England, ch. 5.
Newman, G. R. and Hobot, J. A. (1993) Resin Microscopy and On-Section Immu-nocytochemistry, Springer-Verlag, sBerlin, Germany.
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© 1999 Humana Press Inc.
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Davies, H.A. (1999). General Preparation of Material and Staining of Sections. In: Nasser Hajibagheri, M.A. (eds) Electron Microscopy Methods and Protocols. Methods in Molecular Biology™, vol 117. Humana Press. https://doi.org/10.1385/1-59259-201-5:1
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DOI: https://doi.org/10.1385/1-59259-201-5:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-640-6
Online ISBN: 978-1-59259-201-2
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