Abstract
Cultured human epithelium (autografts) have been used su+ccessfully since 1980 as a permanent cover for large full skin thickness burn wounds (1,1. They were also applied successfully to treat traumatic scars, giant nevi, and tattoo (3,1). The use of allogenic cultured epithelium (allografts) in deep partial and full thickness skin burns has also been used successfully as temporary covering material (5,6). However, the data on the permanent coverage of full thickness skin lesions with allografts are particularly controversial.
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References
O’Connor, N. E., Mulliken, J. B., Banks-Schlegel, S., Kehinde, O., and Green, H., (1981) Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1, 75–78.
Gallico, G. G., O’Connor, N. E., Compton, C. C., Kehinde, O., and Green, H.(1981) Permanent coverage of large burn wounds with autologous cultured human epithelium. N. Engl. J. Med. 311, 448–451.
Gallico, G. G., O’Connor, N. E., Compton, C. C., Remensnyder, J. P., Kehinde O., and Green, H. (1989) Cultured epithelial autografts for giant congenital naevi.Plast. Reconstr. Surg. 84, 1–9.
Carter, D. M., Lin, A. N., Varghese, M. C., Caldwell, D., Pratt, L. A., Eisinger, M.(1987) Treatment of junctional epidermolysis bullosa with epidermal autografts.J. Am. Acad. Dermatol. 172, 246–250.
Thivolet, J., Faure, M., Demidem, A., and Mauduit, G. (1996) Cultured human epidermal allografts are not rejected for a long period. Arch. Dermatol. Res. 278, 252–254.
Phillips, T. J., Kehinde, O., Green, H., and Gilchrest, G. A. (1989) Treatment of skin ulcers with cultured epidermal allografts. J. Am. Acad. Dermatol. 21, 191–199.
Ueda, M. (1995) Formation of epithelial sheets by serially cultivated human mucosal cells and their applications as a graft material. Nagoya J. Med. Sci. 58, 13–28.
Ueda, M., Sumi, Y., and Hata, K. (1999) Establishment of bio-skin bank: how we do it in our hospitals. Ann. Plast. Surg. 42, 574–576.
Teepe, R. G. C., Ponec, R. W., and Hermans, R. P. (1986) Improved grafting method for treatment of burns with autologous cultured human epithelium. Lancet 1, 385.
Langdon, R. C., Cuono, C. B., Birchall, N., Madri, J. A., Kuklinska, E., McGuire, J., and Moellman, G. E. (1988) Reconstruction of structure and cell function in human skin grafts derived from cryopreserved allogeneic dermis and autologous cultured keratinocytes. J. Invest. Dermatol. 81, 478–485.
Rheinwald, J. G. and Green, H. (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cell. Cell 6, 331–344.
Hibino, Y., Hata, K., Horie, K., Torii, S., and Ueda, M. (1996) Structural changes and cell viability of cultured epithelium after freezing storage. J. Craniomaxillofac.Surg. 4, 346–351.
Burt, A. M., Pallett, C. D., Sloane, J. P., et al. (1989) Survival of cultured allografts in patients with burns assessed with probe specific for Y chromosome. BMJ 298, 915–917.
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Ueda, M., Sumi, Y., Hibino, Y., Hata, KI. (2002). Transplantation of Cultured Epithelial Cells. In: Wise, C. (eds) Epithelial Cell Culture Protocols. Methods In Molecular Medicine™, vol 188. Humana Press. https://doi.org/10.1385/1-59259-185-X:197
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DOI: https://doi.org/10.1385/1-59259-185-X:197
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