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Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 173)

Abstract

When a beam of neutrons or X-rays encounters a protein in solution, a small portion of the beam will be deflected or “scattered.” The angular dependence of this scattering is related to the structure of the protein. For a solution of randomly oriented proteins, the scattering is concentrated in the vicinity of the direct beam, or zero-angle. Solution scattering is therefore often referred to as small-angle or low-angle scattering. Structural information encoded in the scattering data includes the overall size and shape of the protein. Although this information is relatively low-resolution, it is not limited by the requirement of having crystals and it can be applied to structures with dimensions in the range 10-1000 Å. This range is extremely useful for studies of proteins and the complexes they form.

Keywords

Neutron Experiment Partial Specific Volume Phosphorylase Kinase Catalytic Cleft Hydrophobic Cleft 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  1. 1.Bioscience Division, Los Alamos National LaboratoryLos Alamos
  2. 2.Chemistry Department, University of North CarolinaCharlotte

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