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Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 200)

Abstract

CpG islands are clusters of CpG dinucleotides that can be found in the 5′ region of about half of human genes (1). Methylation of cytosine within the 5′ CpG islands is associated with transcriptional inactivation of the involved gene. Aberrant methylation of CpG islands is an important mechanism of gene inactivation in cancer (2,3) and other states such as aging and age-related diseases (4). Such methylation often involves numerous CpG islands, and emerging data suggests that methylation profiles may be of some utility in disease detection, prognosis, and risk assessment. However, the measurement of DNA methylation abnormalities at multiple gene loci is currently cumbersome, time-consuming, and not easily amenable to automation. Furthermore, the identification of novel gene sequences hypermethylated in cancer is relatively difficult and inefficient using current technology (5,6). Methylated CpG island Amplification (MCA) was developed to overcome these problems (7).

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Aberrant Methylation SmaI Site Representational Difference Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  1. 1.Graduate School of Biomedical SciencesMD Anderson Cancer CenterHouston

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