Abstract
Enzymatic sequencing of oligosaccharides gives structural information on sequence of monosaccharides and type of linkage within the oligosaccharide chain. This data can be obtained by stepwise enzymatic digestion of a single, isolated oligosaccharide using different mixtures of specific exoglycosidases. N-glycans have to be fractionated from mixtures prior to sequence analysis.
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Kannicht, C., Flechner, A. (2002). Enzymatic Sequence Analysis of N-Glycans. In: Kannicht, C. (eds) Posttranslational Modifications of Proteins. Methods in Molecular Biology™, vol 194. Humana Press. https://doi.org/10.1385/1-59259-181-7:063
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DOI: https://doi.org/10.1385/1-59259-181-7:063
Publisher Name: Humana Press
Print ISBN: 978-0-89603-678-9
Online ISBN: 978-1-59259-181-7
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