Abstract
When performing a structural analysis of N-glycans, a number of aspects should be considered. N-Glycans may be hydrolyzed from purified glycoproteins, serum glycoprotein mixtures, or delipidated membrane fractions by chemical hydrolysis using hydrazine or enzymatical hydrolysis using PNGase F.
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© 2002 Humana Press Inc.
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Nuck, R. (2002). Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB). In: Kannicht, C. (eds) Posttranslational Modifications of Proteins. Methods in Molecular Biology™, vol 194. Humana Press. https://doi.org/10.1385/1-59259-181-7:037
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DOI: https://doi.org/10.1385/1-59259-181-7:037
Publisher Name: Humana Press
Print ISBN: 978-0-89603-678-9
Online ISBN: 978-1-59259-181-7
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