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Transgenesis Techniques pp 3–23Cite as

Biomedical and Agricultural Applications of Animal Transgenesis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 180))

Abstract

In 1980, Gordon et al. (1) showed that DNA injected into the pronuclei of single-cell embryos could be incorporated, expressed, and transmitted to the offspring of transgenic mice. Since then, pronuclear injection has become a widely used and invaluable tool for the study of mammalian gene function. The same technique has also been used to generate transgenic livestock (2); however, the proportion of injected and transferred embryos giving rise to transgenic animals is greatly reduced relative to mice (1 to 2% vs 10-25%). Two general disadvantages of pronuclear injection apply equally to all species: unpredictable effects of site of incorporation and transgene copy number on gene expression lead to a requirement for testing multiple lines to ensure appropriate transgene expression, and the technique is restricted to the addition of genetic material.

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Authors and Affiliations

  1. Division of Molecular Biology, Roslin Institute, Roslin, Midlothian, Scotland

    Jim McWhir

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  1. Jim McWhir
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Editors and Affiliations

  1. School of Biosciences, Cardiff University, Cardiff, UK

    Alan R. Clarke

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© 2002 Humana Press Inc.

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McWhir, J. (2002). Biomedical and Agricultural Applications of Animal Transgenesis. In: Clarke, A.R. (eds) Transgenesis Techniques. Methods in Molecular Biology, vol 180. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-178-7:003

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  • DOI: https://doi.org/10.1385/1-59259-178-7:003

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-696-3

  • Online ISBN: 978-1-59259-178-7

  • eBook Packages: Springer Protocols

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