Abstract
As the genome research continues to grow and genomic information avalanches at the speed of light, biological research labs are facing continuous effort to redefine the scale and scope of their research. Large-scale research applications such as high-throughput sequencing and cDNA microarrays are becoming standard tools in most labs, demanding an increasing ability to generate and handle a large amount of highly purified DNA templates (1). As a result, developing protocols that address the rising needs in sample tracking, robotic compatibility, and efficient integration has become a priority.
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References
DeRisi, J. L., Iyer, V. R., and Brown, P. O. (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278, 680–686.
Huang, G. M., Wang, K., Kuo, C., Paeper, B., and Hood, L. (1994) A high-throughput plasmid DNA preparation method. Analyt. Biochem. 223, 35–38.
Wang, K., Gan, L., Boysen, C., and Hood, L. (1995) A microtiter plate based high throughput DNA purification method. Analyt. Biochem. 226, 85–90.
Itoh, M., Carninci, P., Nagaoka, S., Sasaki, N., Okazaki, Y., Ohsumi, T., et al. (1997) Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates. Nucl. Acids Res. 25, 1315–1316.
Engelstein, M., Aldredge, T. J., Madan, D., Smith, J. H., Mao, J. I., Smith, D. R., and Rice, P.W. (1998) An efficient, automatable template preparation for high throughput sequencing. Microb. Comp. Genomics 3, 237–241.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
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© 2002 Humana Press Inc.
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He, L., Wang, K. (2002). A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method. In: Chen, BY., Janes, H.W. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 192. Humana Press. https://doi.org/10.1385/1-59259-177-9:411
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DOI: https://doi.org/10.1385/1-59259-177-9:411
Publisher Name: Humana Press
Print ISBN: 978-0-89603-969-8
Online ISBN: 978-1-59259-177-0
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