Abstract
Digoxigenin (DIG) is a plant steroid (390 Da) that can be used as a small, stable label of immunoglobulin G (IgG) molecules. It is a valuable alternative to biotin as the biotin-streptavidin system can sometimes give high backgrounds due, for example, to the presence of biotin-containing enzymes in the sample. There is a range of commercially available mouse and sheep anti-DIG Fab antibody fragments labeled with various enzymes and fluorescent molecules for the detection of the DIG-labeled IgG antibody in many applications (1).
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Reference
Kessler, C. (1991) The digoxigenin-anti-digoxigenin (DIG) technology: a survey of the concept and realization of a novel bioanalytical indicator system. Mol. Cell. Probes 5, 161–205.
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© 2002 Humana Press Inc., Totowa, NJ
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Brian Wisdom, G. (2002). Digoxigenin (DIG) Labeling of IgG Antibody. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:349
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DOI: https://doi.org/10.1385/1-59259-169-8:349
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive