β-Galactosidase Labeling of IgG Antibody
The Escherichia coli β-galactosidase (EC 220.127.116.11) is a large enzyme (465 kDa) with a high turnover rate and wide specificity. Unlike several other enzyme labels it is not found in mammalian tissues, hence background contributions from these sources are negligible when this label is measured at a neutral pH.
KeywordsSodium Phosphate Buffer Thiol Group Background Contribution Free Thiol Group Wide Specificity
- 2.Hermanson, G. T. (1996) Bioconjugate Techniques, Academic Press, San Diego, pp. 229–248.Google Scholar