β-Galactosidase Labeling of IgG Antibody

  • G. Brian Wisdom


The Escherichia coli β-galactosidase (EC is a large enzyme (465 kDa) with a high turnover rate and wide specificity. Unlike several other enzyme labels it is not found in mammalian tissues, hence background contributions from these sources are negligible when this label is measured at a neutral pH.


Sodium Phosphate Buffer Thiol Group Background Contribution Free Thiol Group Wide Specificity 
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    O’Sullivan, M. J., Gnemmi, E., Morris, D., Chieregatti, G., Simmonds, A. D., Simmons, M., et al. (1979) Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates to enzyme immunoassay. Analyt. Biochem. 100, 100–108.PubMedCrossRefGoogle Scholar
  2. 2.
    Hermanson, G. T. (1996) Bioconjugate Techniques, Academic Press, San Diego, pp. 229–248.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • G. Brian Wisdom
    • 1
  1. 1.School of Biology and BiochemistryThe Queen’s University, Medical Biology CentreBelfastUK

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