Protein Blotting of Basic Proteins Resolved on Acid-Urea-Triton-Polyacrylamide Gels
The electrophoretic resolution of histones on acetic acid-urea-Triton (AUT) polyacrylamide gels is the method of choice to separate basic proteins, such as histone variants, modified histone species, and high-mobility group proteins 14 and 17 (1-6 and see Chapters 16 and 17). Basic proteins are resolved in this system on the basis of their size, charge, and hydrophobicity. In previous studies, we analyzed the abundance of ubiquitinated histones by resolving the histones on two-dimensional (AUT into SDS) polyacrylamide gels, followed by their transfer to nitrocellulose membranes, and immunochemical staining of nitrocellulose membranes with an antiubiquitin antibody (7-9). However, transfer of the basic proteins directly from the AUT polyacrylamide gel circumvents the need to run the second-dimension SDS gel and accomplishes the analysis of several histone samples. We have described a method that efficiently transfers basic proteins from AUT polyacrylamide gels to nitrocellulose membranes (10). This method has been used in the immunochemical detection of modified histone, isoforms, and histone H1 subtypes (6,11-13).
KeywordsNitrocellulose Membrane Basic Protein Equilibration Buffer Histone Variant Transfer Buffer
- 4.Waterborg, J. H. (1990) Sequence analysis of acetylation and methylation in two histone H3 variants of alfalfa. J. Biol. Chem. 265, 17, 157–17, 161.Google Scholar
- 5.Davie, J. R. and Delcuve, G. P. (1991) Characterization and chromatin distribution of the H1 histones and high-mobility-group non-histone chromosomal proteins of trout liver and hepatocellular carcinoma. Bio chem. J. 280, 491–497.Google Scholar