Protein Blotting by Electroblotting

  • Mark Page
  • Robin Thorpe


Identification of proteins separated by gel electrophoresis or isoelectric-focusing is often compounded by the small pore size of the gel, which limits penetration by macro-molecular probes. Overcoming this problem can be achieved by blotting the proteins onto an adsorbent porous membrane (usually nitrocellulose or diazotized paper), which gives a mirror image of the gel (1). A variety of reagents can be incubated with the membrane specifically to detect and analyze the protein of interest. Antibodies are widely used as detecting reagents, and the procedure is sometimes called Western blotting. However, protein blotting or immunoblotting is the most descriptive.


Cathodal Side Small Pore Size Transfer Buffer Transfer Apparatus Protein Blotting 
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  1. 1.
    Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354.PubMedCrossRefGoogle Scholar
  2. 2.
    Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed. Blackwell Scientific, Oxford, UK.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

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