Protein Blotting by Electroblotting
Identification of proteins separated by gel electrophoresis or isoelectric-focusing is often compounded by the small pore size of the gel, which limits penetration by macro-molecular probes. Overcoming this problem can be achieved by blotting the proteins onto an adsorbent porous membrane (usually nitrocellulose or diazotized paper), which gives a mirror image of the gel (1). A variety of reagents can be incubated with the membrane specifically to detect and analyze the protein of interest. Antibodies are widely used as detecting reagents, and the procedure is sometimes called Western blotting. However, protein blotting or immunoblotting is the most descriptive.
KeywordsCathodal Side Small Pore Size Transfer Buffer Transfer Apparatus Protein Blotting
- 2.Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed. Blackwell Scientific, Oxford, UK.Google Scholar