Difference Gel Electrophoresis

  • Mustafa Ünlü
  • Jonathan Minden


Proteomics is a relatively newly coined term. It refers to the field of study of the proteome, which was defined for the first time in 1994 as ”the PROTEin products of a genOME“ (1). Part of the attractiveness of proteomics derives from its promise to uncover changes in global protein expression accompanying many biologically relevant processes, such as development, tumourigenesis, and so forth. Because proteins are the effector molecules that carry out most cellular functions, studying proteins directly has clear advantages over and (at least in theory) achieves results that go beyond genomic analyses. Whereas the information contained in the genome is almost always static, spatiotemporal patterns of protein expression are very complex and dynamic due to fluctuations in abundance. This complexity is increased several-fold by proteins’ ability to be modified functionally through posttranslational modifications.


Couple Charge Device Camera Teflon Membrane Tile Mode Global Protein Expression Cool Couple Charge Device Camera 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mustafa Ünlü
    • 1
  • Jonathan Minden
    • 1
  1. 1.Millennium PharmaceuticalsCambridge

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