Abstract
Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides not only the ability to resolve and quantify thousands of proteins, but it also gives those in research and industry the ability to monitor in-process protein purification, quickly and easily N. Bizios, unpublished date, (AGI Dermatics). 2-DE is also used to identify variability in protein expression in a variety of cell lines (2,3). It is known that many parameters and laboratory conditions can influence the resolution of proteins on 2-DE, such as the pH range of carrier ampholytes used, the quality of reagents and equipment used, temperature, voltage, and the skill of the researcher or technician.
References
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© 2002 Humana Press Inc., Totowa, NJ
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Bizios, N. (2002). Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:159
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DOI: https://doi.org/10.1385/1-59259-169-8:159
Publisher Name: Humana Press
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