The Bradford Method for Protein Quantitation

  • Nicholas J. Kruger


A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).


Calcium Phosphate Bradford Assay Reagent Blank Lowry Method Guanidine Hydrochloride 
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  1. 1.
    Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248–254.PubMedCrossRefGoogle Scholar
  2. 2.
    Chial, H. J., Thompson, H. B., and Splittgerber, A. G. (1993) A spectral study of the charge forms of Coomassie Blue G. Analyt. Biochem. 209, 258–266.PubMedCrossRefGoogle Scholar
  3. 3.
    Compton, S. J. and Jones, C. G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Analyt. Biochem. 151, 369–374.PubMedCrossRefGoogle Scholar
  4. 4.
    Congdon, R. W., Muth, G. W., and Splittgerber, A. G. (1993) The binding interaction of Coomassie Blue with proteins. Analyt. Biochem. 213, 407–413.PubMedCrossRefGoogle Scholar
  5. 5.
    Friendenauer, S. and Berlet, H. H. (1989) Sensitivity and variability of the Bradford protein assay in the presence of detergents. Analyt. Biochem. 178, 263–268.CrossRefGoogle Scholar
  6. 6.
    Reade, S. M. and Northcote, D. H. (1981) Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Analyt. Biochem. 116, 53–64.CrossRefGoogle Scholar
  7. 7.
    Stoscheck, C. M. (1990) Increased uniformity in the response of the Coomassie Blue protein assay to different proteins. Analyt. Biochem. 184, 111–116.PubMedCrossRefGoogle Scholar
  8. 8.
    Spector, T. (1978) Refinement of the Coomassie Blue method of protein quantitation. A simple and linear spectrophotometric assay for<0.5 to 50 μg of protein. Analyt. Biochem. 86, 142–146.PubMedCrossRefGoogle Scholar
  9. 9.
    Pande, S. V. and Murthy, M. S. R. (1994) A modified micro-Bradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents, and lipids. Analyt. Biochem. 220, 424–426.PubMedCrossRefGoogle Scholar
  10. 10.
    Zuo, S.-S. and Lundahl, P. (2000) A micro-Bradford membrane protein assay. Analyt. Biochem. 284, 162–164.PubMedCrossRefGoogle Scholar
  11. 11.
    Sedmak, J. J. and Grossberg, S. E. (1977) A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G250. Analyt. Biochem. 79, 544–552.PubMedCrossRefGoogle Scholar
  12. 12.
    Peterson, G. L. (1983) Coomassie blue dye binding protein quantitation method, in Methods in Enzymology, vol. 91 (Hirs, C. H. W. and Timasheff, S. N., eds.), Academic Press, New York.Google Scholar
  13. 13.
    Kirazov, L. P., Venkov, L. G. and Kirazov, E. P. (1993) Comparison of the Lowry and the Bradford protein assays as applied for protein estimation of membrane-containing fractions. Analyt. Biochem. 208, 44–48.PubMedCrossRefGoogle Scholar
  14. 14.
    Wilson, C. M. (1979) Studies and critique of amido black 10B, Coomassie Blue R and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis. Analyt. Biochem. 96, 263–278.PubMedCrossRefGoogle Scholar
  15. 15.
    Zor, T. and Selinger, Z. (1996) Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Analyt. Biochem. 236, 302–308.PubMedCrossRefGoogle Scholar
  16. 16.
    Redinbaugh, M. G. and Campbell, W. H. (1985) Adaptation of the dye-binding protein assay to microtiter plates. Analyt. Biochem. 147, 144–147.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Nicholas J. Kruger
    • 1
  1. 1.Department of Plant SciencesUniversity of OxfordUK

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