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Isolation and Purification of Human Mast Cells and Basophils

  • Protocol
Human Airway Inflammation

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 56))

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Abstract

Mast cells were once thought to represent a single population of highly granulated secretory cells. However, with the development of mast cell isolation techniques for a diverse range of tissues, it became apparent that mast cells from different species and those from different tissues within the same species exhibit variations in their biochemical, histochemical, and functional properties. The functional heterogeneity of mast cells has important implications for studies of the response of mast cells to secretory stimuli, antiallergic drugs, or drugs that have the potential to produce adverse responses.

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Author information

Authors and Affiliations

  1. Department of Dermatology, Medical University of Lübeck, Lübeck, Germany

    Bernhard F. Gibbs

  2. Department of Clinical Biochemistry, Institute of Clinical Science, The Queen’s University of Belfast, Belfast, UK

    Madeleine Ennis

Authors
  1. Bernhard F. Gibbs
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  2. Madeleine Ennis
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Editor information

Editors and Affiliations

  1. National Heart and Lung Institute, Imperial College, London, UK

    Duncan F. Rogers  & Louise E. Donnelly  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Gibbs, B.F., Ennis, M. (2001). Isolation and Purification of Human Mast Cells and Basophils. In: Rogers, D.F., Donnelly, L.E. (eds) Human Airway Inflammation. Methods in Molecular Medicine, vol 56. Humana Press. https://doi.org/10.1385/1-59259-151-5:161

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  • DOI: https://doi.org/10.1385/1-59259-151-5:161

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-923-0

  • Online ISBN: 978-1-59259-151-0

  • eBook Packages: Springer Protocols

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