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Quantitative Detection of Bacterial Lipopolysaccharides in Clinical Specimens

  • Protocol
Meningococcal Disease

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 67))

Abstract

Lipopolysaccharides (LPS) located to the outer leaflet of the outer membrane have been identified as the main common endotoxic component of Gramnegative bacteria (1–3). Although other constituents of the bacterial cell wall, i.e., peptidoglycan, may contribute, LPS is considered to be the single most important constituent of Neisseria meningitidis that induces inflammation in the host (4–12). Neisserial lipopolysaccharides are often referred to as lipooligosaccharides (LOS) owing to the short polysaccharide chains comprising approx 10 sugars or less that are attached to lipid A.

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Author information

Authors and Affiliations

  1. Department of Pediatrics, Norway

    Petter Brandtzaeg

  2. University of Oslo, Norway

    Petter Brandtzaeg, Reidun Øvstebø & Peter Kierulf

  3. Department of Clinical Chemistry, Ullevål University Hospital, Norway

    Reidun Øvstebø & Peter Kierulf

Authors
  1. Petter Brandtzaeg
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  2. Reidun Øvstebø
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  3. Peter Kierulf
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Editor information

Editors and Affiliations

  1. BC Research Institute for Children’s and Women’s Health, Vancouver, BC, Canada

    Andrew J. Pollard MD, PhD

  2. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

    Martin C. J. Maiden PhD

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© 2001 Humana Press Inc., Totowa, NJ

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Brandtzaeg, P., Øvstebø, R., Kierulf, P. (2001). Quantitative Detection of Bacterial Lipopolysaccharides in Clinical Specimens. In: Walker, J.M., Pollard, A.J., Maiden, M.C.J. (eds) Meningococcal Disease. Methods in Molecular Medicine™, vol 67. Humana Press. https://doi.org/10.1385/1-59259-149-3:427

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  • DOI: https://doi.org/10.1385/1-59259-149-3:427

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-849-3

  • Online ISBN: 978-1-59259-149-7

  • eBook Packages: Springer Protocols

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