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Mycobacterium tuberculosis Protocols pp 155–164Cite as

Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 54))

Abstract

Genomic variation in any organism is of interest, because it may influence the phenotype of the organism. Special interest currently focuses on prokaryotic pathogens regarding mutations associated with resistance to therapeutic drugs, as well as those mutations involved in the evolution of the bacillus. The molecular basis of drug resistance in Mycobacterium tuberculosis to the front-line drugs is not mediated via plasmids, but is largely, if not entirely, owing to mutations in specific genes of M. tuberculosis. To date, 13 genes are known to be linked to resistance and many functional mutations have been described in these genes (1,2). However, the katG463 (Arg→Leu) and gyrA95 (Thr→Ser) mutations have no apparent relationship to drug resistance, and the combination of these two polymorphisms has been used to place clinical isolates of the M. tuberculosis complex into three evolutionary distinct genotypic groups (3).

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Author information

Authors and Affiliations

  1. MRC Centre for Molecular Cellular Biology, University of Stellenbosch Medical School, Tygerberg, South Africa

    Thomas C. Victor & Paul D. van Helden

  2. Department of Medical Biochemistry, University of Stellenbosch Medical School, Tygerberg, South Africa

    Thomas C. Victor & Paul D. van Helden

Authors
  1. Thomas C. Victor
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  2. Paul D. van Helden
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Editor information

Editors and Affiliations

  1. St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK

    Tanya Parish

  2. London School of Hygiene and Tropical Medicine, London, UK

    Neil G. Stoker

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© 2001 Humana Press Inc., Totowa, NJ

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Victor, T.C., van Helden, P.D. (2001). Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy. In: Parish, T., Stoker, N.G. (eds) Mycobacterium tuberculosis Protocols. Methods in Molecular Medicine, vol 54. Humana Press. https://doi.org/10.1385/1-59259-147-7:155

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  • DOI: https://doi.org/10.1385/1-59259-147-7:155

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-776-2

  • Online ISBN: 978-1-59259-147-3

  • eBook Packages: Springer Protocols

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