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Mycobacterium tuberculosis Protocols pp 59–75Cite as

Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 54))

Abstract

Gene replacement and transposon mutagenesis are two complementary tools that have been widely used to perform genetic studies in various living organisms. In mycobacteria, and especially in the Mycobacterium tuberculosis complex, the lack of these tools has severely hampered the genetic studies of these organisms, especially regarding the identification and characterization of virulence genes of these important pathogens.

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Authors
  1. Mary Jackson
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  2. Luis Reinaldo Camacho
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  3. Brigitte Gicquel
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  4. Christophe Guilhot
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Editor information

Editors and Affiliations

  1. St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK

    Tanya Parish

  2. London School of Hygiene and Tropical Medicine, London, UK

    Neil G. Stoker

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© 2001 Humana Press Inc.

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Cite this protocol

Jackson, M., Reinaldo Camacho, L., Gicquel, B., Guilhot, C. (2001). Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB . In: Parish, T., Stoker, N.G. (eds) Mycobacterium tuberculosis Protocols. Methods in Molecular Medicine, vol 54. Humana Press. https://doi.org/10.1385/1-59259-147-7:059

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  • DOI: https://doi.org/10.1385/1-59259-147-7:059

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-776-2

  • Online ISBN: 978-1-59259-147-3

  • eBook Packages: Springer Protocols

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