Abstract
The multiprobe ribonuclease protection assay (RPA) is a highly sensitive and specific method for simultaneous detection and quantification of several species of mRNA. Three most distinct advantages of the multiprobe RPA method are: high sensitivity and specificity, capacity to simultaneously quantify more than a dozen different mRNA transcripts in a single sample of total RNA, and the flexibility of multiple probes (cDNA, antisense RNA, oligo probe, etc.) that can be used for hybridization with mRNA (Table 1). This method allows one to compare relative mRNA levels of multiple genes, or to compare changes in mRNA levels among various treatments or among various tissues. For such comparisons, the results can be normalized based on the amount of mRNA for housekeeping genes.
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Wang, ZM., Dziarski, R. (2001). Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay. In: O’Neill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicine™, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:125
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DOI: https://doi.org/10.1385/1-59259-146-9:125
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