Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells

  • Jan Eggermont
  • Dominique Trouet
  • Gunnar Buyse
  • Rudi Vennekens
  • Guy Droogmans
  • Bernd Nilius
Part of the Methods in Pharmacology and Toxicology book series (MIPT)


The availability of ion-channel cDNAs has greatly increased our insight in the structure, function, pharmacology, and regulation of ion channels at the molecular level. Much of this knowledge has been obtained by expressing wild-type or mutant ion channels in a heterologous host system, thereby facilitating functional approaches and analyses, which are not possible, when the native channel is studied in its in situ context (1).


Green Fluorescent Protein Internal Ribosomal Entry Site Green Fluorescent Protein Expression Transcription Unit Green Fluorescent Protein Fluorescence 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Jan Eggermont
    • 1
  • Dominique Trouet
    • 1
  • Gunnar Buyse
    • 1
  • Rudi Vennekens
    • 1
  • Guy Droogmans
    • 1
  • Bernd Nilius
    • 1
  1. 1.Laboratory of PhysiologyCatholic University of LeuvenLeuvenBelgium

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