Abstract
DNA analysis methods have been greatly developed in the past 10 yr with the progress of the Human Genome Project (1). In addition to rapid and high-throughput DNA sequencers (2), various reagents (3), and sample preparation methods (4-5)have been developed as well. In order to suit for large scale DNA sequencing, the following methods have been proposed and widely applied in a sequencing facility: shotgun method (6), nested deletion method (7), primer walking method (8), and modular primer method (9)
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Li, T., Okano, K., Kambara, H. (2001). Selective Primer Sequencing from a DNA Mixture by Capillary Electrophoresis. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology™, vol 163. Humana Press. https://doi.org/10.1385/1-59259-116-7:317
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DOI: https://doi.org/10.1385/1-59259-116-7:317
Publisher Name: Humana Press
Print ISBN: 978-0-89603-765-6
Online ISBN: 978-1-59259-116-9
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