Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems

  • Vida Senkus Melvin
  • Dean P. Edwards
Part of the Methods in Molecular Biology™ book series (MIMB, volume 176)


Human progesterone receptor (PR) is a member of the nuclear hormone receptor superfamily of transcriptional activators, which share a common modular structure consisting of a C-terminal ligand-binding domain (LBD), a highly conserved and centrally located DNA-binding domain (DBD), and a poorly characterized N-terminal domain that is required for maximal transcriptional activity (1,2) Human PR is expressed as two proteins from a single gene by alternate use of two promoters: PR-A, which is missing the first 164 amino acids in the N-terminus, and full-length PR-B (3).


Progesterone Receptor Nonspecific Protein Binding Human Progesterone Receptor Ptac Promoter Enterokinase Cleavage Site 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Vida Senkus Melvin
    • 1
  • Dean P. Edwards
    • 1
  1. 1.Department of PathologyUniversity of ColoradoHealth Sciences CenterDenver

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