Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex

  • Rabindra N. Bhattacharjee
  • Caroline Underhill
  • Joseph Torchia
Part of the Methods in Molecular Biology™ book series (MIMB, volume 176)


Nuclear hormone receptors belong to a large family of structurally related proteins that include the steroid, retinoic acid, and vitamin D receptors. These receptors function as ligand-activated transcription factors and regulate complex programs of gene expression involved in growth and differentiation of many tissues. Recent studies have established that binding of hormone to nuclear hormone receptors induces a conformational change in the receptor, which facilitates the recruitment of transcriptional coactivator proteins.


Ammonium Sulfate Wash Buffer Affinity Column Equilibration Buffer Nuclear Hormone Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Halachmi, S., Marden, E., Martin, G., Mackay, I., Abbondanza, C., and Brown, M. (1994) Estrogen receptor-associated proteins: possible mediators of hormoneinduced transcription. Science 264, 1455–1458.PubMedCrossRefGoogle Scholar
  2. 2.
    Kamei, Y., Xu, L., Heinzel, T., Torchia, J., Kurokawa, R., McInerney, E. M., et al. (1996) A CBP Integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors. Cell 85, 403–414.PubMedCrossRefGoogle Scholar
  3. 3.
    Voegel, J. J., Heine, M. J., Zechel, C., Chambon, P., and Gronemeyer, H. (1996) TIF2,a 160 kDa transcriptional mediator for the ligand-dependent activation function of nuclear receptors. EMBO J. 15, 3667–3675.Google Scholar
  4. 4.
    Hong, H., Kohli, K., Trivedi, A., Johnson, D. L., and Stallcup, M. R. (1996) GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors. Proc. Natl. Acad. Sci. USA 93, 4948–4952.PubMedCrossRefGoogle Scholar
  5. 5.
    Torchia, J., Rose, D. W., Inostroza, J., Kamei, Y., Westin, S., Glass, C. K., and Rosenfeld, M. G. (1997) Transcriptional co-activator p/CIP binds CBP and mediatesnuclear-receptor function. Nature 387, 677–684.PubMedCrossRefGoogle Scholar
  6. 6.
    Anzick, S. L, Kononen, J., Walker, R. L., Azorsa, D. O., Tanner, M. M., Guan, X. Y., et al. (1997) AlB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Nature 277, 965–968.Google Scholar
  7. 7.
    Chen, H., Lin, R. J., Schiltz, R. L., Chkravarti, D., Nash, A., Nagy, L., et al. (1997) Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with p/CAF and CBP/p300. Cell 90, 569–580.PubMedCrossRefGoogle Scholar
  8. 8.
    Li, H., Gomes, P. J., and Chen, J. D. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2. Proc. Natl. Acad. Sci. USA 94, 8479–8484.Google Scholar
  9. 9.
    Takeshita, A., Cardona, G. R., Koibuchi, N., Suen, C. S., and Chin, W. W.(1997) TRAM-1, A novel 160-kDa thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1. J. Biol. Chem. 272, 27,629–27,634.PubMedCrossRefGoogle Scholar
  10. 10.
    Suen, C. S., Berodin, T. J., Mastroeni, R., Cheskis, B. J., Lyttle, C. R., and Frail, D. E. (1998) A transcriptional coactivator, steroid receptor coactivator-3, selectively augments steroid receptor transcriptional activity. J. Biol. Chem. 273, 27,645–27,653.PubMedCrossRefGoogle Scholar
  11. 11.
    Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475–1489.PubMedCrossRefGoogle Scholar
  12. 12.
    Harlow, E. and Lane, D. (1999) Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Rabindra N. Bhattacharjee
    • 1
  • Caroline Underhill
    • 1
  • Joseph Torchia
    • 2
  1. 1.London Regional Cancer CentreLondonOntario
  2. 2.Department of Oncology, Pharmacology and ToxicologyUniversity of Western Ontario and the London Regional Cancer CentreOntarioLondon

Personalised recommendations