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Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex

  • Rabindra N. Bhattacharjee
  • Caroline Underhill
  • Joseph Torchia
Part of the Methods in Molecular Biology™ book series (MIMB, volume 176)

Abstract

Nuclear hormone receptors belong to a large family of structurally related proteins that include the steroid, retinoic acid, and vitamin D receptors. These receptors function as ligand-activated transcription factors and regulate complex programs of gene expression involved in growth and differentiation of many tissues. Recent studies have established that binding of hormone to nuclear hormone receptors induces a conformational change in the receptor, which facilitates the recruitment of transcriptional coactivator proteins.

Keywords

Ammonium Sulfate Wash Buffer Affinity Column Equilibration Buffer Nuclear Hormone Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Rabindra N. Bhattacharjee
    • 1
  • Caroline Underhill
    • 1
  • Joseph Torchia
    • 2
  1. 1.London Regional Cancer CentreLondonOntario
  2. 2.Department of Oncology, Pharmacology and ToxicologyUniversity of Western Ontario and the London Regional Cancer CentreOntarioLondon

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