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Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors

  • Frank Wüst
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 176)

Abstract

Nature has made extensive use of metals in biological systems. Metals perform a wide variety of essential biological functions, such as oxygen and electron transfer, the development of structural framework, and they represent the reactive centers in catalytic proteins. The investigation of the biological role of metals and their coordination chemistry in a biosystem is the subject of bioinorganic chemistry (1,1)

Keywords

Steroid Hormone Receptor Pasteur Pipet Pressure Tube Sample Vial Polypropylene Syringe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Lippard, S. J. and Berg, J. M. (1994) Principles of Bioinorganic Chemistry. University Science Books.Google Scholar
  2. 2.
    Garner, C. D.(1997) Bioinorganic chemistry: a personal perspective. J. Chem. Soc. Dalton Trans. 21, 3903–3908.CrossRefGoogle Scholar
  3. 3.
    Orvig, C. and Abrams, M. J. (1999) Medicinal inorganic chemistry: introduction. Chem. Rev. 99, 2201–2203.PubMedCrossRefGoogle Scholar
  4. 4.
    McCarthy T. J., Schwarz S. W., and Welch M. J. (1994) Nuclear medicine and positron emission tomography: an overview. J. Chem. Educ. 71, 830–836.CrossRefGoogle Scholar
  5. 5.
    Johannsen, B. and Spies, H.(1996) Chemistry of technetium(V) as relevant to nuclear medicine. Topics Curr. Chem. 176, 77–121.Google Scholar
  6. 6.
    Katzenellenbogen, J. A. (1993) Molecular probes for steroid receptors: affinity labels, imaging agents and fluorescent ligands, in Trends in Receptor Research (Claassen, V., ed.), Elsevier, pp. 243–257.Google Scholar
  7. 7.
    Katzenellenbogen, J. A. (1995) Designing steroid receptor-based radiotracers to image breast and prostate cancer. J. Nucl. Med. 36(Suppl.), 8S–13S.Google Scholar
  8. 8.
    Hom, R. K. and Katzenellenbogen J. A. (1997) Technetium-99m-labeled receptor-specific small-molecule radiopharmaceuticals: recent developments and encouraging results. Nucl. Med. Biol. 24, 485–498.PubMedCrossRefGoogle Scholar
  9. 9.
    Johannsen, B. and Spies, H. (1997) Advances in technetium chemistry towards 99mTc receptor imaging agents. Transition Metab. Chem. 22, 318–320.CrossRefGoogle Scholar
  10. 10.
    DiZio, J. P., Fiaschi, R., Davison, A., Jones, A. G., and Katzenellenbogen, J. A. (1991) Progestin-rhenium complexes: metal-labeled steroids with high receptor binding affinity, potential receptor-directed agents for diagnostic imaging or therapy. Bioconjugate Chem. 2, 353–366.CrossRefGoogle Scholar
  11. 11.
    DiZio, J. P., Anderson, C. J., Davison, A., Ehrhardt, G. J., Carlson, K. E., Welch, M. J., and Katzenellenbogen J. A. (1992) Technetium-and rhenium-labeled progestins: synthesis, receptor binding and in vivo distribution of an 11β-substituted progestin labeled with technetium-99 and rhenium-186. J. Nucl. Med. 33, 558–569.PubMedGoogle Scholar
  12. 12.
    O’Neil, P. J., Carlson, K. E., Anderson, C. J., Welch, M. J., and Katzenellenbogen, J. A. (1994) Progestin radiopharmaceuticals labeled with technetium and rhenium: synthesis, binding affinity, and in vivo distribution of a new progestin N2S2-metal conjugate. Bioconjugate Chem. 5, 182–193.CrossRefGoogle Scholar
  13. 13.
    Wüst, F., Spies, H., and Johannsen, B. (1996) Synthesis of “3+1” mixed-ligand oxorhenium(V) complexes containing modified 3,17β-estradiol. Bioorg. Med. Chem. Lett. 6, 2729–734.CrossRefGoogle Scholar
  14. 14.
    Reisgys, M., Wüst, F., Alberto, R., Schibli, R., Schubiger, P. A., Pietzsch, H.-J., Spies, H., and Johannsen, B. (1997) Synthesis of rhenium(I) and technetium(I) carbonyl/dithioether ligand complexes bearing 3,17β-estradiol. Bioorg. Med. Chem. Lett. 7, 2243–2246.CrossRefGoogle Scholar
  15. 15.
    Wüst, F., Scheller, D., Spies, H., and Johannsen B. (1998) Synthesis of oxorhenium( V) complexes derived from 7α-functionalized testosterone: first rheniumcontaining testosterone derivatives. Eur. J. Inorg. Chem. 6, 789–793.CrossRefGoogle Scholar
  16. 16.
    Wüst, F., Carlson, K., E., Katzenellenbogen J. A., Spies H., and Johannsen, B. (1998) Synthesis and binding affinities of new 17α-substituted estradiol-rhenium n+1 mixed-ligand and thioether-carbonyl complexes. Steroids 63, 665–671.PubMedCrossRefGoogle Scholar
  17. 17.
    Wüst, F., Skaddan, M. B., Leibnitz, P., Spies, H., Katzenellenbogen, J. A., and Johannsen, B. (1999) Synthesis of novel progestin-rhenium conjugates as potential ligands for the progesterone receptor. Bioorg. Med. Chem. 7, 1827–1835.PubMedCrossRefGoogle Scholar
  18. 18.
    Skaddan, M. B., Wüst, F., and Katzenellenbogen, J. A., (1999) Synthesis and binding affinities of novel rhenium-containing 7α-substituted estradiol complexes: models for breast cancer imaging agents. J. Org. Chem. 64, 8108–8121.PubMedCrossRefGoogle Scholar
  19. 19.
    Marc, B. Skaddan, M. B., Wüst, F., Jonson, S., Syhre, R., Welch, M. J., Spies, H., and Katzenellenbogen J. A. (2000) Radiochemical synthesis and tissue distribution of Tc-99m-labeled 7α-substituted estradiol complexes. Nucl. Med. Biol. 27, 269–278.CrossRefGoogle Scholar
  20. 20.
    Wenzel, M. and Klinge, C. (1994) Tc-99m markierte östradiol-derivate: synthese, organverteilung und tumor affinität. J. Labelled Compd. Radiopharm. 34, 981–987.CrossRefGoogle Scholar
  21. 21.
    Spradau, T. W. and Katzenellenbogen, J. A. (1998) Preparation of cyclopentadienyltricarbonylrhenium complexes using a double ligand transfer reaction. Organometallics 17, 2009–2017.CrossRefGoogle Scholar
  22. 22.
    Mirza, S. A., Pressler, M. A., Kumar, M., Day, R. O., and Maroney, M. J. (1993) Oxidation of nickel thiolates ligands by dioxygen. Inorg. Chem. 32, 977–987.CrossRefGoogle Scholar
  23. 23.
    Spies, H., Fietz, T., Glaser, M., Pietzsch, H.-J., and Johannsen, B. (1995) The “n+1” concept in the synthesis strategy of novel technetium and rhenium tracers, in Technetium and Rhenium in Chemistry and Nuclear Medicine 4 (Nicolini, M., Mazzi, M., and Bandoli, M., eds.), SGEditoriali, Padova, pp. 243–246.Google Scholar
  24. 24.
    Spradau, T. W., Edwards, W. B., Anderson, C. J., Welch, M. J., and Katzenellenbogen, J. A. (1999) Synthesis and biological evaluation of Tc-99m-cyclopentadienyltricarbonyl-technetium-labeled octreotide. Nucl. Med. Biol. 26, 1–7.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Frank Wüst
    • 1
  1. 1.Institut für Interdisziplinäre Isotopenforschung e. V. an der Universität LeipzigLeipzigGermany

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