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Purification and Conjugation of Type 1 Ribosome-Inactivating Proteins

  • Luigi Barbieri
  • Andrea Bolognesi
  • Fiorenzo Stirpe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 166)

Abstract

Ribosome-inactivating proteins (RIPs, 1) are plant enzymes that damage ribosomes in an irreversible manner. They can be divided into type 1 RIPs, which are single-chain proteins, and type 2 RIPs, which are heterodimeric proteins consisting of an enzymatically active A-chain connected by a disulfide bond to a B-chain. The latter has the properties of a lectin specific for sugars with the galactose conformation and binds to galactosyl-terminated receptors on the cell surface, thus allowing the A-chain to enter the cytoplasm. Consequently, most type 2 RIPs are potent toxins, the best known being ricin, while type 1 RIPs are much less toxic.

Keywords

Sodium Phosphate Buffer Sodium Acetate Buffer Crude Enzyme Preparation Cheese Cloth Sodium Borate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Luigi Barbieri
    • 1
  • Andrea Bolognesi
    • 1
  • Fiorenzo Stirpe
    • 1
  1. 1.Dipartimento di Patologia SperimentaleUniversita degli Studi di BolognaBolognaItaly

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