Purification and Conjugation of Type 1 Ribosome-Inactivating Proteins

  • Luigi Barbieri
  • Andrea Bolognesi
  • Fiorenzo Stirpe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 166)


Ribosome-inactivating proteins (RIPs, 1) are plant enzymes that damage ribosomes in an irreversible manner. They can be divided into type 1 RIPs, which are single-chain proteins, and type 2 RIPs, which are heterodimeric proteins consisting of an enzymatically active A-chain connected by a disulfide bond to a B-chain. The latter has the properties of a lectin specific for sugars with the galactose conformation and binds to galactosyl-terminated receptors on the cell surface, thus allowing the A-chain to enter the cytoplasm. Consequently, most type 2 RIPs are potent toxins, the best known being ricin, while type 1 RIPs are much less toxic.


Sodium Phosphate Buffer Sodium Acetate Buffer Crude Enzyme Preparation Cheese Cloth Sodium Borate Buffer 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Luigi Barbieri
    • 1
  • Andrea Bolognesi
    • 1
  • Fiorenzo Stirpe
    • 1
  1. 1.Dipartimento di Patologia SperimentaleUniversita degli Studi di BolognaBolognaItaly

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