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Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 167)

Abstract

Three fundamental technologies have emerged in genetic analysis that have widespread and immediate benefits. DNA synthesis, fluorescence-based DNA analysis, and the polymerase chain reaction (PCR) are being integrated for a range of applications, from forensics to the understanding and treatment of genetic disease. By combining the use of PCR and DNA sequencing, we now have a clear understanding of the molecular basis for many disease states (1). The technologies used to map and sequence the human genome are also being used in leading research establishments to detect DNA mutations leading to genetic diseases such as muscular dystrophy, cystic fibrosis, HIV, and cancer susceptibility (13). The detection of mutations within many disease-related genes can be automated using fluorescent labeled fragments generated by PCR or cycle sequencing. One of Applied Biosystem’s main goals is to develop genetic analysis technologies to meet the needs of the clinical and research settings of the future.

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  • DOI: 10.1385/1-59259-113-2:153
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References

  1. Human disease research (1996) Biosystems Reporter 224, vol. 2, Applied Biosystems.

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  2. Disease gene discoveries made with ABI PRISM systems (1997) Applied Biosystems.

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  3. Common mutation confers resistance to HIV infection (1998) Biosystems Reporter 29, vol. 1, Applied Biosystems.

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© 2001 Humana Press Inc.

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Watts, D., MacBeath, J.R.E. (2001). Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. In: Graham, C.A., Hill, A.J.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 167. Humana Press. https://doi.org/10.1385/1-59259-113-2:153

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  • DOI: https://doi.org/10.1385/1-59259-113-2:153

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-716-8

  • Online ISBN: 978-1-59259-113-8

  • eBook Packages: Springer Protocols