Abstract
Antisense gene therapy involves the selective inhibition of expression or function of disease-causing genes by treatment with an antisense molecules (RNA or oligodeoxynucleotides) complementary to its target nucleic acids, usually to some specific mRNA molecules or mRNA precursor molecules, at a specific site (1,2) (see also Note 1).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Branch, A. D. (1996) A Hitchhiker’s guide to antisense and nonantisense biochemicalpathways. Hepatology. 24, 1517–1529.
Wu, H., Holcenberg, J. S., Tomich, J., Chen, J., Jones, P. A., Huang, S. H., et al. (1990) Inhibition of in vitro transcription by specific double-stranded oligodeoxyribonucleotides. Gene. 89, 203–209.
Meyers, R. A. (ed.) (1995) Molecular Biology and Biotechnology: A Comprehensive Desk Reference, VCH, New York.
Lewin, B. (1997) Genes VI. Oxford University Press, New York.
Trojan, J., Blossey, B. K., Johnson, T. R., Rudin, S. D., Tykocinski, M., Ilan, J., et al. (1992) Loss of tumorigenicity of rat glioblastoma directed by episome-based antisense cDNA transcription of insulin-like growth factor-I. Proc. Natl. Acad. Sci. USA 89, 4874–4878.
Trojan, J., Thomas, R., Johnson, T. R., Susan, D., Rudin, S. D., Ilan, J., et al. (1993) Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA. Science. 259, 94–97.
Ausubel, F. M., Brent, R., Kingston, R. E., et al. (ed.) (1995) Short Protocols in Molecular Biology, Wiley, New York.
Liu, Y. J., Wang, H., Zhao, J., Ma, J., Wei, L. X., Wu, S. G., et al. (2000) Enhancement of immunogenicity of tumor cells by cotransfection with genes encoding antisense insulin-like growth factor-1 and B7.1 molecules. Cancer Gene Therapy. 7, 1–10.
Lafarge-Frayssinet, C., Duc, H. T., Fraysinnet, C., Sarasin, A., Guo, Y. J. and Trojan, J. (1997) Antisense insulin-like factor-1 transfected into rat hepatoma cell line inhibits tumorigenicity by modulating major histocompatatibility complex I cell surface expression. Cancer Gene Therapy. 4, 276–285.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Liu, Y., Zhao, J., Lu, Y., Trojan, J., Wu, M., Guo, Y. (2000). Antisense IGF-I for Hepatocellular Carcinoma. In: Habib, N.A. (eds) Hepatocellular Carcinoma. Methods in Molecular Medicine™, vol 45. Humana Press. https://doi.org/10.1385/1-59259-079-9:221
Download citation
DOI: https://doi.org/10.1385/1-59259-079-9:221
Publisher Name: Humana Press
Print ISBN: 978-0-89603-785-4
Online ISBN: 978-1-59259-079-7
eBook Packages: Springer Protocols