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Phage Display Technology

Protocols
  • Michael Johns
  • Donald B. Palmer
Part of the Methods in Molecular Medicine book series (MIMM, volume 40)

Abstract

Over the past few years, considerable effort has gone into genetic engineering and manipulation of antibody molecules. One consequence of this research has been the use of filamentous phage as a vehicle on which to display antibody fragments. It is possible to express a variety of molecules on the surface of phage, including peptides and antibody fragments. With regard to antibodies, libraries of both sFv and Fab fragments have been successfully cloned onto the surface of bacteriophages, usually via the N terminal of the gene III product, although expression on the gene VIII product has also been employed to isolate low-affinity antibodies.

Keywords

Antibody Fragment Phage Particle Phage Display Library Filamentous Phage Helper Phage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Michael Johns
    • 1
  • Donald B. Palmer
    • 1
  1. 1.Department of ImmunologyImperial College School of MedicineLondonUK

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