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Flow Cytometric Analysis

  • Paul F. McKay
Part of the Methods in Molecular Medicine book series (MIMM, volume 40)

Abstract

Flow cytometry, as the name suggests, is the analysis of cells (which carry one or more fluorescent labels) moving in a fluid flow (1,2). This technique has become widely used because of the enormous increase in the number and range of specificities of antibodies to cell determinants. Monoclonal and polyclonal antibodies to both murine and human antigens, indeed antibodies that have a fluorochrome covalently attached (and which have already been tested in various assay systems), are readily commercially available.

Keywords

Side Scatter Resuspend Cell Residual Medium Residual Fluid Minor Histocompatibility Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Hercher, M., Mueller, W., and Shapiro, H. M. (1979) Detection and discrimination of individual viruses by flow cytometry. J. Histochem. Cytochem. 27, 350–352.PubMedGoogle Scholar
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    Shapiro, H. M. (1977) Fluorescent dyes for differential counts by flow cytometry: does histochemistry tell us much more than cell geometry? J. Histochem. Cytochem. 25, 976–989.PubMedGoogle Scholar
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    Cairo, M. S., Vandeven, C., Toy, C., Tischler, D., and Sender, L. (1988) Fluorescent cytometric analysis of polymorphonuclear leukocytes in Chediak-Higashi syndrome: diminished C3bi receptor expression (OKM1) with normal granular cell density. Pediatr. Res. 24, 673–676.CrossRefPubMedGoogle Scholar
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    Corver, W. E., Cornelisse, C. J., and Fleuren, G. J. (1994) Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin, and propidium iodide on a standard FACScan. Cytometry 15, 117–128.CrossRefPubMedGoogle Scholar
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    Gruber, R., Reiter, C., and Riethmuller, G. (1993) Triple immunofluorescence flow cytometry, using whole blood, of CD4+and CD8+lymphocytes expressing CD45RO and CD45RA. J. Immunol. Methods 163, 173–179.CrossRefPubMedGoogle Scholar
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    Weyde, J., Wassermann, K., and Schell-Frederick, E. (1997) Analysis of single and double-stained alveolar macrophages by flow cytometry. J. Immunol. Methods 207, 115–123.CrossRefPubMedGoogle Scholar

Further Reading

  1. Shapiro, H. M. (1988) Practial Flow Cytometry, 3rd ed., Wiley-Liss Inc., New York. The Cytometry Journal.Google Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Paul F. McKay
    • 1
  1. 1.Department of Viral Pathogenesis, Beth Israel Deaconess Medical Center and Department of MedicineHarvard Medical SchoolBoston

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