How to Set Up an ELISA

  • Bill Jordan
Part of the Methods in Molecular Medicine book series (MIMM, volume 40)


The enzyme-linked immunosorbent assay (ELISA) represents a simple and sensitive technique for specific quantitative detection of molecules to which an antibody is available (1,2). Although there are a huge number of variations based on the original ELISA principle, this chapter will focus on the two perhaps most useful and routinely performed: the indirect sandwich ELISA, providing high sensitivity and specificity; and the basic direct ELISA, useful when only one antibody to the sample antigen is available.


Fetal Calf Serum Detection Antibody Indirect ELISA Capture Antibody Coating Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Kemeny, D. M. and Challacombe, S. J. (1988) ELISA and Other Solid Phase Imunoassays. Theory and Practical Aspects, Wiley, Chichester, UK.Google Scholar
  2. 2.
    Kemeny, D. M. (1991) A Practical Guide to ELISA. Permagon, Oxford, UK.Google Scholar
  3. 3.
    Self, C. H. (1985) Enzyme amplification-a general method applied to provide an immunoassisted assay for placental alkaline phosphatase. J. Immunol. Methods 76, 389–393.CrossRefPubMedGoogle Scholar
  4. 4.
    Bronstein, I., et al. (1993) Chemiluminescent assay of alkaline phosphatase applied in an ultrasensative enzyme immunoassay of thyrotropin. Clin. Chem. 35, 1441–1446.Google Scholar
  5. 5.
    Cook, D. B. and Self, C. H. (1993) Determination of one-thousandth of an attomole (1 zeptomole) of alkaline phosphatase: application in an immunoassay of proinsulin. Clin. Chem. 39, 965–971.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Bill Jordan
    • 1
  1. 1.Department of ImmunologyImperial College School of MedicineLondonUK

Personalised recommendations