Purification of Monoclonal Antibodies Using Protein A/G
A major breakthrough in immunology came with the discovery that large amounts of relatively pure monoclonal antibodies (mAbs) could be prepared from the fusion of B cells (secreting the relevant mAb) with a nonsecreting myeloma cell line (1). Since then mAbs have become a central tool for researchers, enabling them to investigate previously unknown molecules. More recently mAbs have also gained an important role in medicine, e.g., in helping to prevent allograft rejection and in the treatment of digoxin poisoning.
KeywordsHigh Performance Liquid Chromatography Fetal Calf Serum Tissue Culture Medium Tissue Culture Flask Sepharose Bead
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