Purification of Monoclonal Antibodies Using Protein A/G

  • Bridget Heelan
Part of the Methods in Molecular Medicine book series (MIMM, volume 40)


A major breakthrough in immunology came with the discovery that large amounts of relatively pure monoclonal antibodies (mAbs) could be prepared from the fusion of B cells (secreting the relevant mAb) with a nonsecreting myeloma cell line (1). Since then mAbs have become a central tool for researchers, enabling them to investigate previously unknown molecules. More recently mAbs have also gained an important role in medicine, e.g., in helping to prevent allograft rejection and in the treatment of digoxin poisoning.


High Performance Liquid Chromatography Fetal Calf Serum Tissue Culture Medium Tissue Culture Flask Sepharose Bead 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Bridget Heelan
    • 1
  1. 1.Department of ImmunologyImperial College School of MedicineLondonUK

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