Production of Monoclonal Antibodies

  • J’ona Freysd’ottir
Part of the Methods in Molecular Medicine book series (MIMM, volume 40)


The discovery of monoclonal antibodies (mAbs) produced by “hybridoma technology” by George Köhler and Cesar Milstein in 1975 has had a great impact both on basic biological research and on clinical medicine. However, this impact was not immediately recognized. It took around 10 years to appreciate the importance of using these mAbs in various fields of science other than immunology, such as cell biology, biochemistry, microbiology, virology, para-sitology, physiology, genetics, and molecular biology; and also in areas of clinical medicine, such as pathology, hematology, oncology, and infectious disease. The contribution of mAbs to science and clinical medicine was recognized in 1984 by the award of the Nobel Prize for Medicine to Köhler and Milstein.


Spleen Cell Myeloma Cell Counting Chamber Salvage Pathway Myeloma Cell Line 
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Further Reading

  1. Goding, J. W., ed. (1996) Monoclonal Antibodies: Principles and Practice: Production and Application of Monoclonal Antibodies in Cell Biology, Biochemistry and Immunology, 3rd ed., Academic, New York.Google Scholar
  2. Ritter, M. A. and Ladyman, H. M., eds. (1995) Monoclonal Antibodies: Production, Engineering, and Clinical Application, Cambridge University Press, Cambridge, UK.Google Scholar
  3. Springer, T. A., ed. (1985) Hybridoma Technology in the Biosciences and Medicine, Plenum, New York.Google Scholar
  4. McMichael, A. J. and Fabre, J. W., ed. (1982) Monoclonal Antibodies in Clinical Medicine, Academic, New York.Google Scholar
  5. Köhler, G. and Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • J’ona Freysd’ottir
    • 1
  1. 1.Department of Oral MedicineLeeds Dental InstituteLeedsUK

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