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Flow Cytometric Analysis of Hematologic Neoplasia

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Hematologic Malignancies

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 55))

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Abstract

Flow cytometry (FCM) is a powerful technology that allows the rapid analysis of cellular components such as surface and intracellular antigens, or DNA content. The measurements are fast and are based on optical signals emitted by cells labeled with fluorochromes as they flow suspended in a liquid medium through an intense laser beam. The signals include scattered light, which provides information on cell size and granularity, and fluorescence derived from dyes or fluorochrome-labeled antibodies bound to specific cell components.

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Authors and Affiliations

  1. Shands Hospital at the University of Florida College of Medicine, Gainesville, FL

    Raul C. Braylan & John B. Anderson

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  1. Raul C. Braylan
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  2. John B. Anderson
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Editors and Affiliations

  1. VA Medical Center and the Medical College of Georgia, Augusta, GA

    Guy B. Faguet

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© 2001 Humana Press Inc.

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Braylan, R.C., Anderson, J.B. (2001). Flow Cytometric Analysis of Hematologic Neoplasia. In: Faguet, G.B. (eds) Hematologic Malignancies. Methods in Molecular Medicine™, vol 55. Humana Press. https://doi.org/10.1385/1-59259-074-8:217

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  • DOI: https://doi.org/10.1385/1-59259-074-8:217

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-543-0

  • Online ISBN: 978-1-59259-074-2

  • eBook Packages: Springer Protocols

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