Abstract
Plasma lipoproteins are a heterogeneous population of soluble, macromolecular aggregates of lipids and proteins. They are responsible for the transport of waterinsoluble nutrients through the vascular and extravascular fluids from their site of synthesis or absorption to peripheral tissues (1,2). These hydrophobic nutrients (triacylglycerols [TGs] and cholesteryl esters [CEs]) are delivered from the liver and intestine to other tissues in the body for storage or catabolism in the production of energy. Lipoproteins are also known to be involved in other biological processes, including coagulation and tissue repair as well as immune reactions (3,4).
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References
Davis, R. A. and Vance, J. E. (1996) Structure, assembly and secretion of lipoproteins, in Biochemistry ofLipids, Lipoproteins and Membranes (Vance, D. E. and Vance, J. E., eds.), Elsevier, New York, pp. 473–483.
Harmony, J. A. K., Aleson, A. L., and McCarthy, B. M. (1986) Lipoprotein structure and function, in Biochemistry and Biology of Plasma Lipoproteins (Scanu, A. M. and Spector, A. A., eds.), Marcel Dekker Inc., New York, pp. 403–430.
Mbewu, A. and Durrington, P. N. (1990) Lipoprotein (a): Structure, properties and possible involvement in thrombogenesis and atherogenesis. Atherosclerosis 85, 1–14.
Durrington, P. N. (1989) Lipoprotein function, in Lipoproteins and Lipids (Durrington, P. N.,ed.), Wright, London, pp. 255–277.
Davis, R. A. (1991) Lipoprotein structure and secretion, in Biochemistry ofLipids, Lipoproteins, and Membranes (Vance, D. E. and Vance, J. E., eds.),Elsevier, New York, pp. 403–426.
Havel, R. J. and Kane, J. P. (1995) Introduction: Structure and metabolism of plasma lipoproteins, in The Metabolic and Molecular Basis of Inherited Disease, vol. 2, (Scriver, C. R., Beaudet, A. L., Sly, W. S., and Valle, D., eds.), McGraw-Hill Inc., New York, pp. 1129–1138.
Mackness, M. I. and Durrington, P. N. (1992) Lipoprotein separation and analysis for clinical studies, in Lipoprotein Analysis: A Practical Approach (Converse, C. A. and Skinner, E. R., eds.), Oxford University Press, New York, pp. 11–43.
Wasan, K. M., Cassidy, S. M., Ramaswamy, M., Kennedy, A., Strobel, F. A., Ng, S. P., and Lee, T. Y. (1999) A comparison of step-gradient and sequential density ultracentrifugation and the use of lipoprotein deficient plasma controls in determining the plasma lipoprotein distribution of lipid-associated nystatin and cyclosporine. Pharmacol. Res. 16, 165–169.
Schumaker, V. N. and Puppione, D. L. (1986) Lipoprotein Analysis, in Methods of Enzymology (Segrest, J. P. and Albers, J. J., eds.), Academic Press, London, pp. 155–174.
Takayama, M., Itoh, S., and Tanimizu, I. (1977) A new enzymatic method for determination of serum choline-containing phospholipids. Clinica Chim. Acta. 79, 93–98.
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© 2001 Humana Press Inc., Totowa, NJ
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Wasan, K.M., Cassidy, S.M., Kennedy, A.L., Peteherych, K.D. (2001). Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation. In: Drew, A.F. (eds) Atherosclerosis. Methods in Molecular Medicine™, vol 52. Humana Press. https://doi.org/10.1385/1-59259-073-X:27
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DOI: https://doi.org/10.1385/1-59259-073-X:27
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