Abstract
The term interphase cytogenetics was first used in 1986 by Cremer (1,2) to describe detection of chromosomal alterations using in situ techniques in interphase nuclei. This was a distinct advantage over conventional analyses as solid tumors could now be studied (2,3) and the possibility of applying the technique to specimens received in a diagnostic laboratory realised (4). The nonradioactive in situ method, fluorescence in situ hybridization (FISH), has been widely applied to study chromosomal rearrangements, malignancies, and chromosome mapping (4).
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References
Hopman, A. H. N., Ramaekers, F. C. S., Raap, A. K., Beck, J. L. M., Devilee, P., van der Ploeg, M., and Vooijs, G. P. (1988) In situ hybridisation as a tool to study numerical chromosome aberrations in solid bladder tumors. Histochem. 89, 307ā316.
Wolfe, K. Q. and Herrington, C. S. (1997) Interphase cytogenetics and pathology: a tool for diagnosis and research. Mini review. J. Pathol. 181, 359ā361.
Mark, H. F. L., Jenkins, R., and Miller, W. A. (1997) Current applications of molecular cytogenetic technologies. Review. Ann. Clin. Lab. Sci. 27, 47ā56.
Price, C. M. (1993) Fluorescence in situ hybridisation. Blood Rev. 7, 127ā134.
Hopman, A. H. N., van Hooren, E., van de Kaa, C. A., Vooijs, P. G. P., and Ramaekers, F. C. S. (1991) Detection of numerical chromosome aberrations using in situ hybridization in paraffin sections of routinely processed bladder cancers. Mod. Pathol. 4, 503ā513.
Waldman, F. M., Carroll, P. R., Kerschmann, R., Cohen, M. B., Field, F. G., and Mayall, B. H. (1991) Centromeric copy number of chromosome 7 is strongly correlated with tumour grade and labelling index in human bladder cancer. Canc. Res. 51, 3807ā3813.
Macoska, J. A., Micale, M. A., Sakr, W. A., Benson, P. D., and Wolman, S. R. (1993) Extensive genetic alterations in prostate cancer revealed by dual PCR and FISH analysis. Genes, Chromos. Cancer 8, 88ā97.
Sauter, G., Deng, G., Moch, H., Kerschmann, R., Matsumara, K., De Vries, S., etal. (1994) Physical deletion of the p53 gene in bladder cancer. Detection by fluorescence in situ hybridization. Am. J. Pathol. 144, 756ā766.
Sauter, G., Moch, H., Carroll, P., Kerschmann, R., Mihatsch, M. J., and Waldman, F. M. (1995) Chromosome-9 loss detected by fluorescence in situ hybridization in bladder cancer. Int. J. Cancer 64, 99ā103.
Murphy, D. S., McHardy, P., Coutts, J., Mallon, E. A., George, W. D., Kaye, S. B., et al. (1995) Interphase cytogenetic analysis of erbB2 and topoIIār co-amplification in invasive breast cancer and polysomy of chromosome 17 in ductal carcinoma in situ. Int. J. Cancer 64, 18-26.
Pycha, A., Mian, C., Haitel, A., Hofbauer, J., Wiene, H., and Marberger, M. (1997) Fluorescence in situ hybridization identifies more aggressive types of primary non invasive (stage pTa) bladder cancer.J. Urol. 157, 2116ā2119.
Wolman, S. R. (1994) Fluorescence in situ hybridization; a new tool for the pathologist. Human Pathol. 25, 586ā590.
Pahphlatz, M. M. M., de Wilde, P. C. M., Poddighe, P., Dekken, H. V., Vooijs, G. P., and Hanselaar, A. G. J. M. (1995) A model for evaluation of in situ hybridisation spot-count distributions in tissue sections. Cytometry 20, 193ā202.
Visscher, D. W., Wallis, T., and Ritchie, C. A. (1995) Detection of chromosome aneuploidy in breast lesions with fluorescence in situ hybridization: comparison of whole nuclei to thin tissue sections and correlation with flow cytometric DNA analysis. Cytometry 21, 95ā100.
Coignet, L. J. A., Van de Rijke, F. M., Vrolijk, J., Bertheas, M. F., Raap, A. K., and Tanke, H. J. (1996) Automated spot counting of in situ hybridization dots in interphase cells of leukemia samples. Leukemia 10, 1065ā1071.
Bartlett, J. M. S., Watters, A. D., Ballantyne, S. A., Going, J. J., Grigor, K. M., and Cooke, T. G. (1998) Quantitative FISH: is chromosome 9 loss a marker of disease recurrence in TCC of the bladder? Brit. J. Cancer, 77, 2193ā2198.
Warford, T. (1994) An overview of in situ hybridisation, in A Guide to in situ, Hybaid Ltd., UK.
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Watters, A.D., Bartlett, J.M.S. (2000). Quantitation of FISH Signals in Archival Tumors. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicineā¢, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:253
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DOI: https://doi.org/10.1385/1-59259-071-3:253
Publisher Name: Humana Press
Print ISBN: 978-0-89603-583-6
Online ISBN: 978-1-59259-071-1
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