Abstract
Chromosome microdissection is a recently developed molecular cytogenetic technique that has become increasingly important as a bridge connecting cytogenetics to molecular genetics. After a decade of effort, this approach has been developed into a useful and reproducible approach for several purposes, including 1) the isolation of DNA from any cytogenetically recognizable region which can be used to generate DNA microclone libraries for molecular analysis and positional cloning (1,2) the generation of fluorescence in situ hybridization (FISH) probes for whole chromosome painting probes (3), and chromosome arm painting probes (4) for cytogenetic study; 3) combined with FISH, microdissection has been applied to detecte virtually any kind of visible chromosome rearrangements (5,6); and more recently, 4) microdissection combined with hybrid selection has been applied to identify genes associated with homogeneously staining regions (HSRs) in human cancers (7,8).
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References
LĆ¼decke, H-J., Senger, G., Claussen, U., and Horsthemke, B. (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature 338, 348ā350.
Kao, F-T. and Yu, J-W. (1991) Chromosome microdissection and cloning in human genome and genetic disease analysis. Proc. Natl. Acad. Sci. USA 88, 1844ā1848.
Guan, X-Y., Meltzer, P. S., and Trent, J. M. (1994) Rapid construction of whole chromosome painting probes by chromosome microdissection. Genomics 22, 101ā107.
Guan, X-Y., Zhang, H., Bittner, M., Jiang, Y., Meltzer, P. S., and Trent, J. M. (1996) Rapid generation of human chromosome arm painting probes (CAPs) by chromosome microdissection. Nature Genet. 12, 10,11.
Meltzer, P. S., Guan, X-Y., Burgess, A., and Trent, J. M. (1992) Rapid generation of region specific probes by chromosome microdissection and their application. Nature Genet. 1, 24ā28.
Guan, X-Y., Meltzer, P. S., Dalton, W. S., and Trent, J. M. (1994) Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection. Nature Genet. 8, 155ā161.
Su, Y., Meltzer, P. S., Guan, X-Y., and Trent, J. M. (1994) Direct isolation of expressed sequences encoded within a homogeneous staining region by chromosome microdissection. Proc. Natl. Acad. Sci. USA 91, 9121ā9125.
Guan, X-Y., Xu, J., Anzick, S. L., Zhang, H., Trent, J. M., and Meltzer, P. S. (1996) Hybrid selection of transcribed sequences from microdissected DNA: isolation of genes within an amplified region at 20q11-q13.2 in breast cancer. Cancer Res. 56, 3446ā3450.
Trent, J. M. and Thompson, F. H. (1987) Methods for chromosome banding of human and experimental tumors in vitro. Methods Enzymol. 151, 267ā279.
Guan, X-Y., Trent, J. M., and Meltzer, P. S. (1993) Generation of band-specific painting probes from a single microdissected chromosome. Human Mol. Genet. 2, 1117ā1121.
Pinkel, D., Landegent, J., Collins, C., Fuscoe, J., Segraves, R., Lucas, J., and Gray, J. (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21and translocation of chromosome 4. Proc. Natl. Acad. Sci. USA 85, 9138ā9142.
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Guan, Xy., Trent, J.M. (2000). Chromosome Microdissection for Detection of Subchromosomal Alterations by FISH. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicineā¢, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:247
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DOI: https://doi.org/10.1385/1-59259-071-3:247
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