Plant Hormone Protocols pp 101-121

Part of the Methods in Molecular Biology™ book series (MIMB, volume 141)

Cytokinins

Extraction, Separation, and Analysis
  • Paula E. Jameson
  • Huaibi Zhang
  • David H. Lewis

Abstrect

Analysis of cytokinins in plant tissues has historically been laborious and time consuming because of the extremely low levels and diverse molecular structures of the cytokinins. The initial anticipation that cytokinins could be quantified by radioimmunoassay of crude plant extracts (1,2) was, unfortunately, not justified. Detailed analysis requires the sample to be well purified and the individual cytokinins to be separated, so that not only is interference in the assay avoided, but also problems associated with differential crossreactivity of individual cytokinins with the antibodies in the immunoassay (3). The (sometimes subtle) differences in the chemical properties of the cytokinin free bases, ribosides, nucleotides, and the O- and N-glucosides enables the ready separation and quantification of the majority of the cytokinins.

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Paula E. Jameson
    • 1
  • Huaibi Zhang
    • 2
  • David H. Lewis
    • 3
  1. 1.Institute of Molecular BioSciences, College of SciencesMassey UniversityPalmerston NorthNew Zealand
  2. 2.The Horticulture and Food Research Institute of New ZealandPalmerston NorthNew Zealand
  3. 3.New Zealand Institute for Coop and Food ResearchPalmerston NorthNew Zealand

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