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Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells

  • Anthony M. De Lise
  • Rocky S. Tuan
Part of the Methods in Molecular Biology™ book series (MIMB, volume 137)

Abstract

Electroporation has been frequently adopted as a means to transfect primary cells or cell lines that demonstrate resistance to other commonly used methods of transfection, e.g., DEAE-dextran or calcium phosphate precipitation or liposome-mediated transfection. Electroporation is a method of transfection utilizing an electric field to introduce foreign materials into cells. This method of transfection has been demonstrated to be effective on both prokaryotic (1, 2, 3, 4) and eukaryotic cells (5, 6, 7, 8) and is capable of introducing DNA, large macromolecules (e.g., antibodies), proteins, dyes, metabolic precursors (e.g., 32P-ATP), and nonpermeant drugs and metabolites into cells with high efficiency (4,8, 9, 10, 11, 12, 13).

Keywords

Transfection Efficiency Tissue Culture Plate Electric Field Pulse Micromass Culture Electroporation Cuvet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Anthony M. De Lise
    • 1
  • Rocky S. Tuan
    • 1
  1. 1.Department of Orthopaedic SurgeryThomas Jefferson UniversityPhiladelphia

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