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Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery

  • David S. PackardJr.
  • Christopher Cox
  • Thomas J.
Part of the Methods in Molecular Biology™ book series (MIMB, volume 137)

Abstract

For more than a century, avian embryos have been used in the study of development. Yet, as research techniques improve, there is increased need for animal models that exhibit excellent development in vitro and are easily accessible for observations, manipulations, and cell culturing. Herein, we describe improved techniques (1) for culturing avian embryos on a transparent substratum, (2) for microsurgery and transplantation of avian embryo tissues, and (3) for the in vitro cultivation of avian somite cells.

Keywords

Culture Dish Stereo Microscope Primitive Streak Vitelline Membrane Avian Embryo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • David S. PackardJr.
    • 1
  • Christopher Cox
    • 1
  • Thomas J.
    • 1
  1. 1.Department of Anatomy and Cell BiologyState University of New York Health Science CenterSyracuse

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