Abstract
During early craniofacial development, cranial neural crest cells emigrate from seg-mentally distinct divisions of the hindbrain (rhombomeres) to populate the various branchial arches and subsequently differentiate into multiple neuronal and non-neu-ronal cell lineages of the head and neck region. Organ culture of the mandibular portion of the developing first branchial arch (1,2) facilitates investigations of molecular signaling events of crest cell differentiation during chondrogenesis, osteogenesis, odontogenesis, and myogenesis (3-5). In addition, organs explanted in culture are assessable physically by microdissections, implantations, transplantations, and micro-injections, and can be readily manipulated by antisense oligonucleotides strategies, viral delivery, and immunoperturbation methodologies.
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References
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Slavkin, H., Nuckolls, G., Shum, L. (2000). Craniofacial Development and Patterning. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols: Volume II. Methods in Molecular Biology™, vol 136. Humana Press. https://doi.org/10.1385/1-59259-065-9:45
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DOI: https://doi.org/10.1385/1-59259-065-9:45
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