Liquid Chromatographic Method for Aflatoxin M1 in Milk

  • Hans P. van Egmond
  • Sylviane Dragacci
Part of the Methods in Molecular Biology™ book series (MIMB, volume 157)


Aflatoxin M1 is the 4-hydroxy derivative of aflatoxin B1 (see Fig. 1). Aflatoxin M1 appears in milk and milk products as the direct result of the intake of aflatoxin B1 -contaminated feed by dairy cows (1). The amount excreted as aflatoxin M1, as a percentage of aflatoxin B1 in feed, is usually 1-4% (2), but values as high as 6% have been reported at μg daily intake levels of aflatoxin B1(3). The carry-over of aflatoxin B1 to milk may vary largely from animal to animal, from day to day, and from one milking to the next.
Fig. 1.

Chemical structure of aflatoxin M1.


High Performance Liquid Chromatography High Performance Liquid Chromatography Milk Sample Phase High Performance Liquid Chromatography Immunoaffinity Column 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Hans P. van Egmond
    • 1
  • Sylviane Dragacci
    • 2
  1. 1.EU Community Reference Laboratory for ResiduesNational Institute of Public Health and the EnvironmentThe Netherlands
  2. 2.EU Community Reference Laboratory for Milk and Milk Products, French Agency for Food SafetyMaisons-AlfortCedexFrance

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