Electron Cryomicroscopy of Fibrillar Collagens
Collagen fibrils in tissue are generally heterotypic with more than one type of collagen molecule incorporated into the fibril structure. Furthermore specific macromolecules are bound onto the fibril surface influencing both the assembly and the interaction of the fibril with the surrounding matrix. The electron cryomicroscopy procedures described here form part of a program of work to determine the structure and assembly of tissue fibrils containing surface-associated components.
KeywordsCollagen Fibril Stereo Pair Agar Scientific Contrast Transfer Function Formvar Film
- 9.Sherratt, M., Graham, H. K., Kielty, C. M., and Holmes, D. F. (1999) ECM mac-romolecules: rotary shadowing and scanning transmission electron microscopy. Methods in Molecular Biology, Extracellular Matrix Protocols (Streuli, C. and Grant, M., ed.), Humana Press, Totowa, NJ.Google Scholar