Purification of GroES from an Overproducing E. coliStrain

  • Elsie Quaite-Randall
  • Andrzej Joachimiak
Part of the Methods in Molecular Biology™ book series (MIMB, volume 140)


Of the Hsp10/cpn10 family of molecular chaperones, GroES, the cochaperonin or “release factor” from Escherichia coli is the best characterized. Since GroEL and GroES are on the same operon in E. coli (1), they are usually overexpressed together in the same strain (seeNote 1). We commonly purify GroES from the same cells used for GroEL purification (2).


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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Elsie Quaite-Randall
    • 1
  • Andrzej Joachimiak
    • 1
  1. 1.Center for Mechanistic Biology and Biotechnology, Structure Biology CenterArgonne National LaboratoryArgonne

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