Abstract
Growth factor receptors endowed with intrinsic tyrosine-kinase activity (receptor tyrosine kinases, RTKs) are capable of intracellular signal transduction through their ability to autophosphorylate and to phosphorylate cellular proteins, globally referred to as substrates (refs. 1–3 and references therein).
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References
Schlessinger J. and Ullrich A. (1992) Growth factor signaling by receptor tyrosine kinases. Neuron 9, 383–391.
Fantl W. J., Johnson D. E., and Williams L. T. (1993) Signaling by receptor tyrosine kinases. Annu. Rev. Biochem. 62, 453–481.
Kazlauskas A. (1994) Receptor tyrosine kinases and their targets. Curr. Opin. Genet. Dev. 4 5–14.
Cohen G. B., Ren R., and Baltimore D. (1995) Modular binding domains in signal transduction proteins. Cell 80, 237–248.
van der Geer P. and Pawson T. (1995) The PTB domain: a new protein module implicated in signal transduction. Trends Biochem. Sci. 20, 277–280.
Carpenter G. (1992) Receptor tyrosine kinase substrates: src homology domains and signal transduction. FASEB J. 6, 3283–3289.
Romano A., Wong W. T., Santoro M., Wirth P. J., Thorgeirsson S. S., and Di Fiore P. P. (1994) The high transforming potency of erbB−2 and ret is associated with phosphorylation of paxillin and a 23 kDa protein. Oncogene 9, 2923–2933.
Kanner S. B., Reynolds A. B., and Parsons J. T. (1989) Immunoaffinity purifi-cation of tyrosine-phosphorylated cellular proteins. J. Immunol. Methods 120 115–124.
Glenney J. R. and Zokas L. (1989) Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton. J. Cell. Biol. 108, 2401–2408.
Kanner S. B., Reynolds A. B., Vines R. R., and Parsons J. T. (1990) Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases. Proc. Natl. Acad. Sci. USA 87, 3328–3332.
Glenney J. R. (1991) Isolation of tyrosine-phosphorylated proteins and generation of monoclonal antibodies. Meth. Enzymol. 201, 92–100.
Skolnik E. Y., Margolis B., Mohammadi M., Lowenstein E., Fischer R., Drepps A., et al. (1991) Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 65, 83–90.
Fazioli F., Bottaro D. P., Minichiello L., Auricchio A., Wong W. T., Segatto O., and Di Fiore P. P. (1992) Identification and biochemical characterization of novel putative substrates for the epidermal growth factor receptor kinase. J. Biol. Chem. 267, 5155–5161.
Lowenstein E. J., Daly R. J., Batzer A. G., Li W., Margolis, B., Lammers R., et al. (1992) The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell 70, 431–442.
Schaller M. D. Borgman C. A., Cobb B. S., Vines R. R., Reynolds A. B., and Parsons J. T. (1992) pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA 89, 5192–5196.
Fazioli F., Wong W. T., Ullrich S. J., Sakaguchi K., Appella E., and Di Fiore P. P. (1993) The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation.Oncogene 8, 1335–1345.
Fazioli F., Minichiello L., Matoska V., Castagnino P., Miki T., Wong W. T., and Di Fiore P. P. (1993) Eps8,a substrate for the epidermal growth factor receptor kinase enhances EGF-dependent mitogenic signals. EMBO J. 12 3799–3808.
Fazioli F., Minichiello L., Matoskova B., Wong W. T., and Di Fiore P. P. (1993) eps15, a novel tyrosine kinase substrate, exhibits transforming activity. Mol. Cell.Biol. 13, 5814–5828.
Di Fiore P. P., Pierce J. H., Fleming T. P., Hazan R., Ullrich A., King C. R., et al.(1987) Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells. Cell 51, 1063–1070.
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Di Fiore, P.P., Fazioli, F. (2000). Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:221
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DOI: https://doi.org/10.1385/1-59259-059-4:221
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