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Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries

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Protein Kinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 124))

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Abstract

Growth factor receptors endowed with intrinsic tyrosine-kinase activity (receptor tyrosine kinases, RTKs) are capable of intracellular signal transduction through their ability to autophosphorylate and to phosphorylate cellular proteins, globally referred to as substrates (refs. 13 and references therein).

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© 2000 Humana Press Inc., Totowa, NJ

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Di Fiore, P.P., Fazioli, F. (2000). Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:221

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  • DOI: https://doi.org/10.1385/1-59259-059-4:221

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-700-7

  • Online ISBN: 978-1-59259-059-9

  • eBook Packages: Springer Protocols

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