Abstract
The JAK/STAT pathway is activated by a wide range of ligands including cytokines and growth factors (1,2; see Table 1). This pathway was discovered by two independent approaches that initially identified the role of JAKs and STATs in interferon signaling. The first, a biochemical approach, used promoter elements to isolate and purify STATs (3) and the second, a genetic approach, led to the isolation of mutants defective in their response to interferon (4). The latter resulted in eight mutant cell lines (summarized in Table 2) which were complemented by components of the interferon pathway (5–9). These mutants have been fundamental in demonstrating a role for JAKs and STATs in cytokine signaling and in understanding the mechanisms of activating the JAK/STAT pathway.
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Broughton, N., Burfoot, M.S. (2000). JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins. In: Reith, A.D. (eds) Protein Kinase Protocols. Methods in Molecular Biology™, vol 124. Humana Press. https://doi.org/10.1385/1-59259-059-4:131
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DOI: https://doi.org/10.1385/1-59259-059-4:131
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